Multistep process of neoplastic transformation of normal human fibroblasts by 60CO gamma rays and harvey sarcoma viruses

✍ Scribed by Masayoshi Namba; Koji Nishitani; Fujiko Fukushima; Tetsuo Kimoto; Kiyoshi Nose

• Book ID: 102868684
• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: French
• Weight: 735 KB
• Volume: 37
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910370314

✦ Synopsis

As reported previously (Namba ef a/., 1985), normal human fibroblasts were transformed by %o gammaray irradiation into immortal cells with abnormal karyotypes. These transformed cells (KMST-6), however, showed a low cloning efficiency in soft agar and no transplantability. However, upon treatment with Harvey murine sarcoma virus (Ha-MSV), the cells acquired elevated clonability in soft agar and transplantability in nude mice. Ha-MSV alone, however, did not convert normal human fibroblasts into either immortal or tumorigenic cells. The Ha-MSV-transformed KMST-6 cells showed an enhanced expression of the ras oncogene, but normal and 6oCo gamma-ray-transformed cells did not. Our current data suggest that gamma rays worked against normal human cells as an initiator, giving rise to chromosome aberrations and immortality, and that Ha-MSV, probably through its ras oncogene, played a role in the progression of the malignant cell population to a more malignant one showing enhanced colony formation in soft agar and tumorigenicity in nude mice.