𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Multiplex PCR for identification of herpes virus infections in adolescents

✍ Scribed by Julia Durzyńska; Joanna Pacholska-Bogalska; Maria Kaczmarek; Tomasz Hanć; Magdalena Durda; Magdalena Skrzypczak; Anna Goździcka-Józefiak

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
99 KB
Volume
83
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The aim of the study was to develop a multiplex PCR (mPCR) for a rapid and simultaneous detection of herpes simplex 1 (HSV‐1), herpes simplex 2 (HSV‐2), and human cytomegalovirus (HCMV) DNA in squamous oral cells obtained from adolescents. Accuracy of the method was tested in a group of 513 adolescents, almost 11% of subjects were positive for infection with herpes viruses. Correlations with gender, age, and place of residence were sought. A similar incidence of HSV‐2 and HCMV was found (4.3% and 5.4%, respectively) and the incidence of HSV‐1 was the lowest (1%) in the study group. Conversely to HSV‐2, HCMV was detected mostly in the youngest individuals. The same occurrence of all viruses was observed in boys and girls. The mPCR method described is suggested as a useful tool for epidemiologic studies of active herpes infections. J. Med. Virol. 83:267–271, 2011. © 2010 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Suitability and clinical application of
Suitability and clinical application of a multiplex nested PCR assay for the diagnosis of herpes simplex virus infections
✍ Cassinotti, P.; Mietz, H.; Siegl, G. 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 776 KB

A novel multiplex nested polymerase chain reaction (PCR) assay was designed and evaluated for routine diagnosis of herpes simplex virus (HSV) infections in patients with either putative HSV infection of the central nervous system or suspected HSV keratitis. Single-tube amplification of HSV type 1 (H

The usefulness of multiplex PCR for the
The usefulness of multiplex PCR for the identification of bacteria in joint infection
✍ Hwanjung Kim; Jimyung Kim; Chunhwa Ihm 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 122 KB

## Abstract __Background__: The diagnosis of septic arthritis (SA) relies on synovial analysis and conventional culture. But, these methods lack of sensitivity and culture is time consuming to establish a definite diagnosis. This study evaluated a new multiplex PCR assay which entailed screening PC

Real-time nested multiplex PCR for the d
Real-time nested multiplex PCR for the detection of Herpes simplex virus types 1 and 2 and Varicella zoster virus
✍ Hugh J. O'Neill; Dorothy E. Wyatt; Peter V. Coyle; Conall McCaughey; Frederick M 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 66 KB 👁 1 views

## Abstract One hundred forty‐nine specimens were tested in a LightCycler nested multiplex polymerase chain reaction (LCnmPCR) for Herpes simplex virus (HSV)1, HSV2, and VZV. Eighty‐one were from genitourinary medicine (GUM) patients and the other 68 specimens were from other patients with skin les

Screening for possible failure of herpes
Screening for possible failure of herpes simplex virus PCR in cerebrospinal fluid for the diagnosis of herpes simplex encephalitis
✍ Elisabeth Puchhammer-Stöckl; Elisabeth Presterl; Cornelia Croÿ; Stefan Aberle; T 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 98 KB 👁 1 views

## Abstract The objectives of this study were to evaluate the reliability of herpes simplex virus (HSV) PCR testing in cerebrospinal fluid (CSF) for the detection of herpes simplex encephalitis. This was done by examining retrospectively the clinical follow‐up of a large group of patients tested ro

Identification of viral and atypical bac
Identification of viral and atypical bacterial pathogens in children hospitalized with acute respiratory infections in Hong Kong by multiplex PCR assays
✍ R.Y.T. Sung; Paul K.S. Chan; Tracy Tsen; A.M. Li; W.Y. Lam; Apple C.M. Yeung; E. 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 123 KB

## Abstract Acute respiratory tract infection is a leading cause of hospital admission of children. This study used a broad capture, rapid and sensitive method (multiplex PCR assay) to detect 20 different respiratory pathogens including influenza A subtypes H1, H3, and H5; influenza B; parainfluenz

Comparison of multiplex PCR assays and c
Comparison of multiplex PCR assays and conventional techniques for the diagnostic of respiratory virus infections in children admitted to hospital with an acute respiratory illness
✍ François Freymuth; Astrid Vabret; Delphine Cuvillon-Nimal; Sandrine Simon; Julia 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 115 KB

The performances of four multiplex PCR (m-PCR) were compared to direct immunofluorescence assay (DFA) and HuH7 cell culture for the detection of viruses in 263 children admitted to hospital with an acute respiratory illness. One hundred fifty (57.6%) nasal aspirates were found DFApositive; 188 (72.3