Multiplex detection of KRAS mutations by a matrix-assisted laser desorption/ionization-time of flight mass spectrometry assay
β Scribed by Choi, Hee Sook; Min, Kyung Tae; Cha, Yoon Seok; Hong, Sun Pyo
- Book ID
- 122272791
- Publisher
- Elsevier Science
- Year
- 2014
- Tongue
- English
- Weight
- 915 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0009-9120
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A nonradioactive assay for protein tyrosine phosphatases (PTPs), employing a tyrosine-phosphorylated peptide as a substrate, has been developed and applied to analyze purified enzymes, cell extracts, and immunoprecipitates. The reaction was followed by matrix-assisted laser desorption ionization tim
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was applied to the detection of mutations involving single nucleotides. For detection of a single-nucleotide deletion, a normally 44 bp region of the L1CAM gene was amplified in a 50 microL solution, and measu