Multiplex bacterial growth monitoring in 24-well microplates using a dual optical sensor for dissolved oxygen and pH
✍ Scribed by Anna S. Kocincová; Stefan Nagl; Sarina Arain; Christian Krause; Sergey M. Borisov; Matthias Arnold; Otto S. Wolfbeis
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 417 KB
- Volume
- 100
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Abstract
Non‐invasive, simultaneous optical monitoring of oxygen and pH during bacterial cultivation in 24‐well microplates is presented using an integrated dual sensor for dissolved oxygen and pH values. The dual sensor is based on oxygen‐sensitive organosilica microparticles and pH‐sensitive microbeads from a polymethacrylate derivative embedded into a polyurethane hydrogel. The readout is based on a phase‐domain fluorescence lifetime‐based method referred to as modified frequency domain dual lifetime referencing using a commercially available detector system for 24‐well microplates. The sensor was used for monitoring the growth of Pseudomonas putida bacterial cultures. The method is suitable for parallelized, miniaturized bioprocessing, and cell‐based high‐throughput screening applications. Biotechnol. Bioeng. 2008;100: 430–438. © 2008 Wiley Periodicals, Inc.