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Multiplex and Real-Time Quantitative PCR on Fetal DNA in Maternal Plasma: A Comparison with Fetal Cells Isolated from Maternal Blood

✍ Scribed by SINUHE HAHN; XIAO YAN ZHONG; MARTIN R. BÜRK; CAROLYN TROEGER; WOLFGANG HOLZGREVE

Book ID: 111396489
Publisher: John Wiley and Sons
Year: 2006
Tongue: English
Weight: 165 KB
Volume: 906
Category: Article
ISSN: 0890-6564
DOI: 10.1111/j.1749-6632.2000.tb06605.x

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Fetal cells are present in the circulation of pregnant women and can be isolated using density gradient centrifugation and magnetic cell sorting. In the present study, maternal cell preparations were depleted for CD45and CD14-positive cells and enriched for CD71-positive cells. The number of fetal n