Multiplex amplification and detection of single nucleotide polymorphisms in cytokine loci
β Scribed by Valeria Brizzio; Phillis Ottaviano; Ivan Balazs
- Book ID
- 117414988
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 23 KB
- Volume
- 63
- Category
- Article
- ISSN
- 0198-8859
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This study introduces a DNA microarray-based genotyping system for accessing single nucleotide polymorphisms (SNPs) directly from a genomic DNA sample. The described one-step approach combines multiplex amplification and allele-specific solid-phase PCR into an on-chip reaction platform. The multiple
## Abstract With costβeffective highβthroughput Single Nucleotide Polymorphism (SNP) arrays now becoming widely available, it is highly anticipated that SNPs will soon become the choice of markers in whole genome screens. This optimism raises a great deal of interest in assessing whether dense SNP