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Multiple transformation of Saccharomyces cerevisiae by protoplast fusion

✍ Scribed by Emer Reeves; Kevin Kavanagh; Peter A. Whittaker

Book ID
109317900
Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
379 KB
Volume
99
Category
Article
ISSN
0378-1097

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πŸ“œ SIMILAR VOLUMES

Multiple fusion of protoplasts in Saccha
Multiple fusion of protoplasts in Saccharomyces yeasts
✍ Arima, Kenji ;Takano, Isamu πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 610 KB

Fusion of protoplasts prepared from haploid strains of Saccharomyces yeasts having identical mating type was induced with the aid of polyethylene glycol. Stable fusion products were isolated by complementation of the auxotrophic markers. Of 64 isolates derived by protoplast fusion between two differ

Sporulation of products of protoplast fu
Sporulation of products of protoplast fusion without regeneration in Saccharomyces cerevisiae
✍ Tsuboi, Michio πŸ“‚ Article πŸ“… 1981 πŸ› Springer 🌐 English βš– 554 KB

In Saccharomyces cerevisiae, diploid strains which are respiratory deficient (e.g., rho-) or are homozygous for the mating-type locus (i.e., either a/a or c~/~) are unable to sporulate. In order to induce sporulation in these nonsporulating strains, the technique of protoplast fusion mediated by pol