Multiple second messenger pathways regulate IL-β-induced expression of PGHS-2 mRNA in normal human skin fibroblasts
✍ Scribed by Lorna Jane Warnock; Gary W. Hunninghake
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 806 KB
- Volume
- 163
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
Very little is known about the specific regulation of PGHS-2 mRNA compared with PGHS-1 mRNA. Using normal human fibroblasts, we show that at baseline there is constitutive expression of PGHS-1 mRNA and barely detectable amounts of PGHS-2 mRNA. There was a marked increase in PGHS-2 mRNA transcription following exposure to IL-1 p. Maximal expression of PGHS-2 mRNA occurred with concentrations of IL-1 p 3 1 ngiml at 3 hours after stimulation. Downregulation of protein kinase C (PKC) activity by pretreating fibroblast cultures with PMA inhibited IL-1-induced PCHS-2 mRNA expression without affecting the constitutive expression of PGHS-1 mRNA. The addition of various PKC inhibitors also blocked the IL-l p induction of PGHS-2 mRNA but did not alter PGHS-1 mRNA expression; inhibitors of protein kinase A (PKA) or tyrosine kinase (TK) had only a limited effect on I L -l p-induced PCHS-2 mRNA expression. These findings show that IL-lp increases PGHS-2 mRNA, at least in part, via activation of PKC.