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Multiple-pulse electroporation: Uptake of a macromolecule by individual cells of Saccharomyces cerevisiae

✍ Scribed by R.E. Brown; D.C. Bartoletti; G.I. Harrison; T.R. Gamble; J.G. Bliss; K.T. Powell; J.C. Weaver

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
770 KB
Volume
28
Category
Article
ISSN
1567-5394

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Saccharomyces cerevisiae strain 83384-B3 carries the sai-1 mutation which confers sensitivity to S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH). It was shown that the mutant is impermeable to precursors of ribonucleic acid (RNA) and protein during inhibition by SAM (0.2 mM). Inhibition