Multiple-Particle Tracking Measurements of Heterogeneities in Solutions of Actin Filaments and Actin Bundles
โ Scribed by Apgar, Joshua; Tseng, Yiider; Fedorov, Elena; Herwig, Matthew B.; Almo, Steve C.; Wirtz, Denis
- Book ID
- 118513549
- Publisher
- Biophysical Society
- Year
- 2000
- Tongue
- English
- Weight
- 495 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0006-3495
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The diffusion coefficients of monodisperse polystyrene latex spheres in solutions of polymerized actin were measured using dynamic light scattering. Four different probes with radii R, ranging from 50 to 500 nm, were separately used in actin solutions with concentrations c, ranging from 1.5 to 21 p
We study the polymerization kinetics and linear rheology of actin filaments in the absence and in the presence of latrunculin A. Filamentous actin is a semiflexible polymer, and latrunculin A is an organic, actin-binding molecule. Using diffusing wave spectroscopy (DWS), we monitor the thermally exc