Multiple forms of wheat leaf N-acetyl-β-d-hexosaminidase
✍ Scribed by N.D. Jordan; M.S. Barber
- Book ID
- 119137461
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 690 KB
- Volume
- 107
- Category
- Article
- ISSN
- 0168-9452
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## Abstract The degradative enzyme N‐acetyl‐β‐D‐hexosaminidase (Hex) (E.C. 3.2.1.30) has been isolated from livers and tail fins of the amphibian __R. catesbeiana__. Two amphibian hexosaminidases have been resolved by polyacrylamide gel electrophoresis and quantitated by assay of the separated form
K,-cells from Drosophila melanogaster, grown under serum-free conditions, produce two p-hexosaminidases and secrete these enzymes into the medium. The two enzymes were separated by DEAE-exchange chromatography. According to their substrate specificities one enzyme is a f3-N-acetyl-D-glucosarninidase
Kc-cells from Drosophila produce two different beta-D-hexosaminidases, a beta-N-acetyl-D-glucosaminidase (E.C.3.2.1.30) and a beta-N-acetyl-D-hexosaminidase (E.C.3.2.1.52), which are also secreted into the medium. The Mr of both enzymes is about 126,000 +/- 9,700; the S-values are 8.37 +/- 0.44. Bot