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Multiple forms of porcine pancreatic phospholipase A2: Isolation and specificity

✍ Scribed by Tsao, Francis H. C. ;Cohen, Herschel ;Snyder, William R. ;Kézdy, Ferenc J. ;Law, John H.

Publisher: Wiley (John Wiley & Sons)
Year: 1973
Tongue: English
Weight: 420 KB
Volume: 1
Category: Article
ISSN: 0091-7419
DOI: 10.1002/jss.400010605

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✦ Synopsis

Abstract

Porcine pancreatic phospholipase A~2~ was purified from commercial pancreatin by a method involving heat denaturation, trichloroacetic acid precipitation, and DEAE‐cellulose chromatography. Assaying the eluate of the chromatography step by a new titrimetric method using vegetable lecithin‐albumin emulsion as the substrate, several species of phospholipase A were found. Some of these went undetected when the conventional egg yolk emulsion assay was used. Two phospholipases A~2~ were isolated in a homogeneous form and shown to have similar chemical and physical properties. Catalytic specificity of the two enzymes differs remarkably toward lecithins in different emulsified states.

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