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Multiple forms of acid phosphatase from seedling axes of Vigna radiata

✍ Scribed by Pratima De-Kundu; Ambika C. Banerjee


Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
353 KB
Volume
29
Category
Article
ISSN
0031-9422

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An acid phosphatase from derepressed cells of the yeast form of Yarrowia lipolyrica was purified 176-fold by ammonium sulfate precipitation, chromatography on DEAE-Sephacel and gel filtration in Biogel A 5-M. The enzyme is a glycoprotein with a sugar content of 60%. The MICHAELIS constant of the enz