Multiple Alternating Molecular Layers of Albumin and Heparin on Solid Surfaces

✍ Scribed by Eduard Brynda; Milan Houska

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 107 KB
• Volume: 183
• Category: Article
• ISSN: 0021-9797
• DOI: 10.1006/jcis.1996.0514

✦ Synopsis

The grafting of hydrophilic, neutral, highly mobile Films consisting of various numbers of alternating molecular polymer chains, such as poly(oxyethylene), on the surface layers of human serum albumin (HSA) and heparin were prepared prevents adsorption and denaturation of plasma proteins and on surfaces of polyethylene, polyurethane, cellulose acetate, gerconsequent platelet adhesion and fibrin deposition. The more manium, hydrophilic and hydrophobic silica, and mica by alternatmobile the polymer chains are the better the thromboresistiving the HSA and heparin adsorption from solutions at pH 3.9.

ity of the surface (3). According to the hydrated surface

The successive adsorption of the molecular layers was observed dynamics hypothesis the rapid movement of the hydrated in situ by IR multiple internal reflection spectroscopy and total chains repulses the blood components (4).

internal reflection fluorescence. The first HSA monolayer was irre-(ii) The treatment of surfaces with albumin solutions is versibly adsorbed on the substrates mainly due to the hydrophobic interaction with the surface, and the following heparin and HSA often done prior to clinical use of artificial kidneys and blood layers were successively bound due to ionic interaction between oxygenators. Albumin adsorbs irreversibly on most solid HSA and heparin. Films stable in physiological solutions were surfaces due to the hydrophobic dehydration or combination obtained by the covalent crosslinking of the final HSA-heparin of hydrophobic and electrostatic interactions. The preadsorpassembly.