## Abstract A novel twoβdimensional electrophoretic system for the control of electroosmosis in capillary zone electrophoresis has been developed and evaluated for rapid separations of proteins. The system comprises uncoated and polyetherβcoated fused silica capillaries coupled in series. An equati
Multiple advantages of capillary zone electrophoresis for exploring protein conformational stability
β Scribed by Daniel Rochu; Patrick Masson
- Book ID
- 102681406
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 1003 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0173-0835
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## Detection of unwanted protein-bound ligands by capillary zone electrophoresis: The case of hidden ligands that stabilize cholinesterase conformation Detection, identification and characterization of compounds present in purified proteins and biopharmaceuticals are of central interest. As well a
Non-native conformations of proteins were generated by temporary contact with aqueous solutions of sodium dodecyl sulfate (SDS) and separated from the native state with capillary zone electrophoresis (CZE) in alkaline borate buffer deficient of SDS. Nine proteins at concentrations of 2.0 or 3.0 mg.L