Multiple activation of chloroform in kidney microsomes from male and female DBA/2J mice
✍ Scribed by Ade, Paola ;Guastadisegni, Cecilia ;Testai, Emanuela ;Vittozzi, Luciano
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 616 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0887-2082
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✦ Synopsis
Microsomes from the renal cortex of DBA/2J mice can metabolize chloroform through oxidative and reductive pathways, similar to hepatic microsomes.
The oxidative or reductive nature of CHC13 activation is strictly dependent on the oxygenation of the incubation mixture, as indicated by the formation of qualitatively different adducts to phospholipids (PLs). The protein and lipid binding levels measured in kidney microsomes from control females differed significantly from the binding levels observed with kidney microsomes from male and testosteronetreated female DBA/2J mice in aerobic conditions only. Therefore, the sex-dependent CHC13-induced acute nephrotoxicity seems related only with the oxidative CHC13 activation. The levels of adducts to PL polar heads and to protein showed a strict correlation with each other. Therefore, the assay of adducts to PL polar heads may be used as a substitute for the assay of adducts to protein. This might be especially convenient when studying the effects of both phosgene and the trichloromethyl radicals.