Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe
✍ Scribed by Gangjun Liu; Khanh Kieu; Frank W. Wise; Zhongping Chen
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 447 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1864-063X
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✦ Synopsis
Abstract
We report on the development of a compact multiphoton microscopy (MPM) system that integrates a compact and robust fiber laser with a miniature probe. The all normal dispersion fiber femtosecond laser has a central wavelength of 1.06 μm, pulse width of 125 fs and average power of more than 1 W. A double cladding photonic crystal fiber was used to deliver the excitation beam and to collect the two‐photon signal. The hand‐held probe included galvanometer‐based mirror scanners, relay lenses and a focusing lens. The packaged probe had a diameter of 16 mm. Second harmonic generation (SHG) images and two‐photon excited fluorescence (TPEF) images of biological tissues were demonstrated using the system. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)