Multiparametric study of atresia in ewe antral follicles: Histology, flow cytometry, internucleosomal DNA fragmentation, and lysosomal enzyme activities in granulosa cells and follicular fluid
✍ Scribed by Ana María Rosales-Torres; Alejandro Avalos-Rodríguez; Marcela Vergara-Onofre; Omar Hernández-Pérez; Luz María Ballesteros; Rebeca García-Macedo; Vianney Ortíz-Navarrete; Adolfo Rosado
- Book ID
- 101267957
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 200 KB
- Volume
- 55
- Category
- Article
- ISSN
- 1040-452X
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✦ Synopsis
The differential quantitative participation of apoptosis and necrosis in ewe antral follicles of two different sizes, separated in four stages of atresia using macroscopic, histologic, and esteroid quantification methods was assessed. Annexin V binding and propidium iodide (PI) uptake was used to detect healthy live cells (Annexin V negative/PI negative), early apoptotic cells (Annexin Vϩ/PIϪ), and necrotic or late apoptotic cells (PIϩ). Additionally we used internucleosomal DNA fragmentation as a quantitative estimate of apoptosis. Presence and distribution of lysosomal enzymes in follicular fluid and granulosa cells was used as a measure of necrotic cell death. DNA flow citometry and gel electrophoresis were positively correlated with the progression of atresia, small atretic follicles tend to have higher percentages of internucleosomal cleaved DNA than follicles Ͼ6 mm. Annexin/PI binding also indicates that apoptosis and necrosis increase with atresia progression, generally apoptosis outweighs necrosis in small follicles. Acid phosphatase and glucosaminidase in follicular fluid of 3-6 mm follicles showed no significant modifications between healthy and initially atretic follicles, and only a small, but significant increase in activity in advancedly atretic follicles. On the contrary, lysosomal enzyme activity in follicles Ͼ6 mm showed positive correlation between atresia stages and the activities of acid phosphatase and glucosaminidase in follicular fluid. A similar sizedifferential behavior was found in free or membranebound lysosomal enzyme activity of granulosa cells. Necrosis, but principally apoptosis, were present during all stages of follicular maturation indicating that growth and maturation of ovarian follicles involves a continuous renewal of granulosa cells, regulated by apoptosis. Mechanisms regulating this equilibrium may participate in the final destiny, whether ovulation or