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Multiparameter Imaging for the Analysis of Intracellular Signaling

✍ Scribed by Carsten Schultz; Andreas Schleifenbaum; Joachim Goedhart; Theodorus W. J. Gadella Jr.

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
301 KB
Volume
6
Category
Article
ISSN
1439-4227

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

In biological experimentation and especially in drug discovery there is a trend towards more complex test systems. Cell‐based assays are replacing conventional binding or enzyme assays more and more. This development is strongly driven by novel fluorescent probes that give insight into cellular processes. Target proteins are studied in their natural environment; this gives much more realistic test results, especially with respect to enzyme location and kinetics. However, in the complex environment of cells, many parameters contribute to the performance of the protein of interest. Therefore, it would be desirable to monitor simultaneously as many of the relevant cellular processes as possible. Here, we discuss the possibilities and limitations provided by multiparameter monitoring of cellular events with fluorescent probes. Some novel examples of the use of fluorescent probes and multiparameter imaging are shown.

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