Apoptosis is a vital process for organism development and, when disrupted, can lead to abnormalities including cancer and autoimmune diseases. We demonstrate a novel multicolor flow cytometry approach for quantifying apoptosis and cell cycle information of phenotypically distinct populations, using
Multiparameter flow cytometric approach for simultaneous evaluation of T lymphocyte-endothelial cell interactions
โ Scribed by Alexander S. Krupnick; Daniel Kreisel; Wilson Y. Szeto; Sicco H. Popma; Kunjlata M. Amin; Jonni S. Moore; Bruce R. Rosengard
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 315 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0196-4763
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## Abstract Coโimmunoprecipitation is the classical approach for investigating proteinโprotein interactions. Analysis is generally conducted using the Western blot approach. We set out to investigate whether flow cytometry was a feasible alternative to Western blotting. Using the TCRโCD3 complex as