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Multimodal biophotonic workstation for live cell analysis

✍ Scribed by Michael Esseling; Björn Kemper; Maciej Antkowiak; David J. Stevenson; Lionel Chaudet; Mark A. A. Neil; Paul W. French; Gert von Bally; Kishan Dholakia; Cornelia Denz

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
281 KB
Volume
5
Category
Article
ISSN
1864-063X

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✦ Synopsis

Abstract

A reliable description and quantification of the complex physiology and reactions of living cells requires a multimodal analysis with various measurement techniques. We have investigated the integration of different techniques into a biophotonic workstation that can provide biological researchers with these capabilities. The combination of a micromanipulation tool with three different imaging principles is accomplished in a single inverted microscope which makes the results from all the techniques directly comparable. Chinese Hamster Ovary (CHO) cells were manipulated by optical tweezers while the feedback was directly analyzed by fluorescence lifetime imaging, digital holographic microscopy and dynamic phase‐contrast microscopy. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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