Multilayer immobilized-enzyme filter reactors: Urease bound to nylon fabric filters

✍ Scribed by Lev Shemer; Roni Granot; Amihay Freeman; Mordechai Sokolovsky; Leon Goldstein

• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: English
• Weight: 774 KB
• Volume: 21
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260210908

✦ Synopsis

Abstract

Urease was bound to commercially available nonwoven nylon fabric filters. Multilyer immoibilized‐enzyme filter reactors were constructed by packing varying numbers of urease‐nylon filters in a column. Owing to the relatively open structure and high mechanical strength of the filter fabric, compaction and pressure drop effects were minimal. The reactors could be operated in a wide range of substrate concentrations and flow rates under conditions where mass‐transfer limitations could be neglected. The kinetic behavior of the immobilized‐enzyme filter reactors could be described by a linear form of the integrated Michaelis‐Menten equation using a model based on the sequential action of the enzyme filters.