Multigene expression in vivo: Supremacy of large versus small terminators for T7 RNA polymerase

Engineering multigene expression in vitr

Engineering multigene expression in vitro and in vivo with small terminators for T7 RNA polymerase

✍ Liping Du; Rong Gao; Anthony C. Forster 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 600 KB

## Abstract Engineering protein expression in vitro or in vivo is usually straightforward for single genes, but remains challenging for multiple genes because of the requirement of coordinated control. RNA and protein overexpression strategies often exploit T7 RNA polymerase and its natural TΦ Clas