Multifunctional mesoporous silica nanoparticles as labels for the preparation of ultrasensitive electrochemical immunosensors

✍ Scribed by Minghui Yang; He Li; Alireza Javadi; Shaoqin Gong

• Book ID: 104003959
• Publisher: Elsevier Science
• Year: 2010
• Tongue: English
• Weight: 679 KB
• Volume: 31
• Category: Article
• ISSN: 0142-9612
• DOI: 10.1016/j.biomaterials.2010.01.033

✦ Synopsis

Labels based on mesoporous silica nanoparticles (MSN) loaded with mediator thionine (TH), enzyme horseradish peroxidase (HRP) and secondary anti-human IgG antibody (Ab 2 ) were developed in order to improve the sensitivity and detection limit of an amperometric immunosensor. The sensitivity of the sandwich-type immunosensor using MSN-TH-HRP-Ab 2 as labels for human IgG detection was about 100 times higher than that using either MSN-TH-Ab 2 or MSN-HRP-Ab 2 as labels, indicating the high catalytic efficiency of HRP in the presence of mediator TH toward H 2 O 2 . The immunosensor using MSN-TH-Ab 2 as labels exhibited a high sensitivity and showed a linear response within the range of 0.01-10 ng/mL human IgG. It also showed good reproducibility and selectivity and acceptable stability. These labels for immunosensors may provide many potential applications for the detection of different biomolecules.