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Multicentre Italian Study Group (MISG) for the standardisation of hepatitis C virus (HCV) PCR

โœ Scribed by Carlo Mancini; Marisa Zerbini; Alberta Azzi; Marisa Piunno


Book ID
117769623
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
286 KB
Volume
27
Category
Article
ISSN
1386-6532

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โœ Olmedo, Eva; Costa, Josep; L๏ฟฝpez-Labrador, Fancesc X.; Forns, Xavier; Ampurdan๏ฟฝs ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 169 KB ๐Ÿ‘ 2 views

A modified competitive RT-PCR (mcRT-PCR) to measure HCV RNA in serum and the Amplicor HCV Monitor assay were compared. For mcRT-PCR, the RNA extracted was retrotranscribed and coamplified in one step with a known amount of a DNA internal control (IC). Digoxigenin-labeled amplified products were hybr