Multi-system approach to analysis of T-lymphocyte activation by flow cytometry: Utilization of intracellular cytokine expression, cytokine receptor expression, and quantification of cytokine secretion as an indicator of activation

T-lymphocyte functionality assessed by a

T-lymphocyte functionality assessed by analysis of cytokine receptor expression, intracellular cytokine expression, and femtomolar detection of cytokine secretion by quantitative flow cytometry

✍ Daniel P. Collins; Barbara J. Luebering; David M. Shaut 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 155 KB 👁 2 views

## Assessing the functionality of T lymphocytes is important in determining progression rate in human immunodeficiency virus (HIV) infection and acquired immunodeficiency syndrome (AIDS), transplant rejection, and autoimmune disease. Activation of T-cells in response to antigen results in expressi