๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Multi-stage mass spectrometry for the isolation and structure elucidation of components of a crude extract

โœ Scribed by Strife, Robert J.; Ketcha, Marcia M.; Schwartz, Jae

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
421 KB
Volume
32
Category
Article
ISSN
1076-5174

No coin nor oath required. For personal study only.

โœฆ Synopsis

Multi-stage mass spectrometry (MSn) (up to n = 6) using a combination of electrospray ionization with a quadrupole ion trap was applied to the gas-phase isolation and direct structure elucidation of a complicated surfactant molecule in a crude product extract. Doubly unsaturated ions were found to arise from neutral, saturated fatty amide side chains via a unique fragmentation in the positive ion mode. Negative ion experiments revealed the substructure of the anionic portion of the surfactant. Strategies for software-controlled acquisition and mapping of MSn data are discussed.

๐Ÿ“œ SIMILAR VOLUMES

Ion trap multiple-stage tandem mass spec
Ion trap multiple-stage tandem mass spectrometry as a pre-NMR tool in the structure elucidation of saponins
โœ Dirk C van Setten; Gijsbert Zomer; Gerrit van de Werken; Emmanuel J H J Wiertz; ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 141 KB ๐Ÿ‘ 2 views

Ion trap MS has been utilized in the structure elucidation of a saponin from Quillaja saponaria. With respect to the carbohydrate moieties, this technique allowed the elucidation of the sequence and branching, of six out of seven glycosidic linkages, the precise location of an appended O-acetyl grou

Separation and first structure elucidati
Separation and first structure elucidation of Cremophorยฎ EL-components by hyphenated capillary electrophoresis and delayed extraction-matrix assisted laser desorption/ionization-time of flight-mass spectrometry
โœ Thomas Meyer; Dietmar Waidelich; August Wilhelm Frahm ๐Ÿ“‚ Article ๐Ÿ“… 2002 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 227 KB

The polyethoxylated heterogeneous components of the so far poorly characterized nonionic emulsifier Cremophor EL (polyoxyl 35 castor oil) (CrEL) were fractionated by cyclodextrin-modified micellar electrokinetic capillary chromatography (CD-MEKC). Due to the low UV absorbance of most of the CrEL-com

Photochemistry of threeN-acetoacetyl ami
Photochemistry of threeN-acetoacetyl amino acid methyl esters: structure elucidation of the radiation products by gas chromatography/mass spectrometry
โœ Budzikiewicz, H.; Dallakian, P.; Griesbeck, A. G.; Heckroth, H. ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 115 KB ๐Ÿ‘ 2 views

The photochemistry of three N-acetoacetyl a-amino acid (valine, tert-leucine, isoleucine) methyl esters was investigated. The products after UV irradiation in acetonitrile at 300 nm (direct excitation) were analyzed by gas chromatography coupled with chemical ionization mass spectrometry. In all cas

A comparison of matrix-assisted laser de
A comparison of matrix-assisted laser desorption/ionization time-of-flight and liquid chromatography electrospray ionization mass spectrometry methods for the analysis of crude tarantula venoms in the Pterinochilus group
โœ Pierre Escoubas; Julia Chamot-Rooke; Reto Stรถcklin; Brian J. Whiteley; Gerardo C ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 129 KB ๐Ÿ‘ 2 views

The search for novel pharmacological tools in spider venoms involves the need for precise and reproducible species identification methods. As an addition to morphological analysis, we have developed venom fingerprinting by reversed-phase chromatography and matrix-assisted laser desorption/ionization