Multi-component calibration and analysis in liquid chromatography

✍ Scribed by Todd L. Cecil; Russell B. Poe; Sarah C. Rutan

• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 743 KB
• Volume: 250
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(91)85060-6

✦ Synopsis

Multl-wavelength detectors offer Improved detecbon capablhttes for hqmd chromatographlc methods, but reqmre

Improvements m data analysis methodology to utlhze all the avadable mformatton In ths work, vanous methods for ~mprovmg the quanbtative results obtamed from hqrud chromatography with full spectrum fluorescence detectlon were studled The avadablhty of multi-wavelength mformatlon allows overlapped chromatographx peaks to be resolved Different approaches were mvestigated for developmg cahbratton models that use all of the avadable spectral mformatton, and are compatible wth a vartety of methods for quanttflcatton, mcludmg factor analysts, Kalman fdtenng and rank anndulatlon These methods were compared for the= abdlty to resolve overlapped chromatographlc peaks and then accuracy m quantlficatlon