Multi-analyte miniature conductance biosensor

✍ Scribed by D.C. Cullen; R.S. Sethi; C.R. Lowe

• Book ID: 104100654
• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 682 KB
• Volume: 231
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/s0003-2670(00)86394-1

✦ Synopsis

The fabrication and operation of a multi-analyte miniature conductance hiosensor is described The device responds to changes in the electrode double layer capacitance as the ionic strength is increased by the enzyme-catalysed generation of charged reaction products . Enzymes such as urease and L-asparaginase and a three-enzyme system consisting of urease, creatinase and creatminase were used to determine urea, L-asparagine and creatinine, respectively Careful choice of suitable enzyme immobilization regimes led to device response times for the attainment of the steady state of the order of seconds . This fast response time allowed the construction of a flow cell with the incorporation of a urease-modified device for the continuous measurement of urea in flowing systems