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MSR—A program for kinetic analysis of enzyme modification data on PC

✍ Scribed by Chun Liu; Bao-He Qu

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
505 KB
Volume
23
Category
Article
ISSN
0010-4825

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✦ Synopsis

A computer program has been written for the kinetic method of substrate reaction (MSR) in enzyme modification studies. The program analyses a series of progress curves that continuously record signals (absorbance, fluorescence emission, etc.) reflecting the concentration changes of substrate or product during enzyme modification in the presence of substrate. After the apparent kinetic parameters are calculated by graphdrawing or curve-fitting method, a series of relevant plots is shown, from which information about the enzyme-inhibitor interaction can be deduced and microscopic kinetic constants calculated.

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