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mRNA sequencing of novel cell lines from human papillomavirus type-16 related vulval intraepithelial neoplasia: Consequences of expression of HPV16 E4 and E5

✍ Scribed by Bryant, Dean; Onions, Tiffany; Raybould, Rachel; Flynn, Áine; Tristram, Amanda; Meyrick, Sian; Giles, Peter; Ashelford, Kevin; Hibbitts, Samantha; Fiander, Alison; Powell, Ned


Book ID
124075877
Publisher
John Wiley and Sons
Year
2014
Tongue
English
Weight
184 KB
Volume
86
Category
Article
ISSN
0146-6615

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We have cloned cDNA of the major 1.8 kb mRNA from HPV 16-transformed NIH 3T3 cells (PM3T3). The entire nucleotide sequences of this cDNA were determined and compared with prototype HPV 16 genomic DNA sequences. The 5'-end of the cDNA was flanked by approximately 300 bp of cellular sequences, and the