✍ Scribed by A. Martínez; I. Olarte; M.A. Mergold; M. Gutiérrez; E. Rozen; J. Collazo; O. Amancio-Chassin; R.M. Ordóñez; J.J. Montesinos; H. Mayani; D.K. McCurdy; P. Ostrosky-Wegman; E. Garrido-Guerrero; E.I. Miranda
No coin nor oath required. For personal study only.
Leukemia-associated antigens such as proteins encoded by MAGE genes might provide tools for immunotherapy of leukemia. Positive and negative results of MAGE-A gene expression in hematological malignancies have been reported. This led us to study MAGE-A gene expression in human leukemias using RT-PCR. Among 115 leukemias from various subtypes, 14/34 (41.17%) AML were positive for one of the three genes analyzed (MAGE-A1 1/32; MAGE-A3 10/32; MAGE-B2 3/12). Expression was also detected in 23/76 (30.26%) B-cell ALL patients (MAGE-A1 2/53; MAGE-A3 20/53; MAGE-B2 1/32). One of these patients expressed both MAGE-A1 (weak signal) and -A3 (strong signal) genes. Other patient with CML were positive for MAGE-B2 (1/5, 20%). MAGE-A3 expression data were corroborated by real time RT-PCR through determination of MAGE-A3 transcript levels. We concluded that the MAGE-A3 gene is expressed at the mRNA level in a proportion of human leukemias.
📜 SIMILAR VOLUMES
## Fas (Apo-1/CD95 ) is a cell membrane receptor involved in apoptotic cell death. Soluble variant forms (sFas) lacking the transmembrane domain due to alternative splicing have been identified. Up-regulation of sFas expression is reportedly implicated in prereceptorial blockage of Fas-induced apo
The human MAGE-I, MAGEJ and MART-I genes code for antigens that are specifically recognized by cytolytic T lymphocytes in a MHC-restricted manner. The MAGE-I and MAGEJ genes are expressed in tumors of different histotypes but not in normal adult tissues (with the exception of testis), while the MART