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mRNA display for the selection and evolution of enzymes from in vitro-translated protein libraries

✍ Scribed by Seelig, Burckhard


Book ID
109946894
Publisher
Nature Publishing Group
Year
2011
Tongue
English
Weight
574 KB
Volume
6
Category
Article
ISSN
1750-2799

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A library of mutants of the RTEM 13-1actamase displayed on phage was created; it contained penicillin binding proteins (PBPs) as well as a small fraction of active B-lactamases. The library was submitted to a selection process to extract the B-lactamases i.e. the enzymes that turnover efficiently. T