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MRI/MRS evaluation of cariporide in a canine long-term model of reperfused ischemic insults

✍ Scribed by Kerry Thompson; Gerald Wisenberg; Jane Sykes RVT; R. Terry Thompson


Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
157 KB
Volume
17
Category
Article
ISSN
1053-1807

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✦ Synopsis


Abstract

Purpose

To examine with magnetic resonance imaging (MRI)/magnetic resonance spectroscopy (MRS) the long‐term effects of cariporide in a canine cardiac ischemia/reperfusion model.

Materials and Methods

Twenty‐two beagles underwent a 2‐hour occlusion followed by 10 days of reperfusion. Cine MRI and ^31^P MRS were performed to monitor function and metabolism of the heart in the control (N = 10) and cariporide (N = 12) groups. Radioactively labeled microspheres were injected to determine coronary blood flow, and contrast‐enhanced ex vivo MRI assessed infarct volumes.

Results

Cariporide produced a significant reduction vs. controls, in intracellular pH, during ischemia (P < 0.05) and at days 3 and 10 postreperfusion (P < 0.0005). Functional recovery of the myocardium was significantly improved immediately upon reperfusion (percent of baseline: 63.5% ± 3.5% for controls, 90.5% ± 7.2% for cariporide) and at day 3, but not by day 10. Normalized infarct ratios (IRs) were similar for controls and cariporide (0.58 ± 0.08, 0.58 ± 0.06, respectively).

Conclusion

Cariporide augments early functional recovery, while delaying normalization of intracellular pH following ischemia/reperfusion, but confers neither long‐term functional or metabolic protection nor, most importantly, myocardial salvage. J. Magn. Reson. Imaging 2003;17:136–141. © 2002 Wiley‐Liss, Inc.