𝔖 Bobbio Scriptorium
✦   LIBER   ✦

MRI detection of macrophages labeled using micrometer-sized iron oxide particles

✍ Scribed by John B. Williams; Qing Ye; T. Kevin Hitchens; Christina L. Kaufman; Chien Ho

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
524 KB
Volume
25
Category
Article
ISSN
1053-1807

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Purpose

To evaluate cellular labeling of immune cells using micron‐sized iron oxide particles (MPIOs) and evaluate the MR relaxivity and MRI detection of the labeled cells.

Materials and Methods

Immune cells isolated from mice and rats were labeled with three different sizes of MPIO particles (0.35, 0.90, or 1.63 μm). These labeled cells were characterized using transmission electron microscopy (TEM), fluorescence microscopy, flow cytometry, MR relaxometry, and MRI.

Results

Macrophage uptake of MPIOs was found to be highest for the 1.63‐μm size particles. MR relaxivity measurements indicated greater spin‐spin relaxation for MPIO‐labeled cells relative to cells labeled with nanometer‐sized ultra‐small superparamagnetic iron oxide (USPIO) particles with similar iron content. TEM and fluorescence microscopy indicated cellular uptake of multiple MPIO particles per cell. Macrophages labeled with 1.63‐μm MPIOs had an average cellular iron uptake of 39.1 pg/cell, corresponding to approximately 35 particles per cell.

Conclusion

Cells labeled with one or more MPIO particles could be readily detected ex vivo at 11.7 Tesla and after infusion of the MPIO‐labeled macrophages into the kidney of a rat, hypointense regions of the outer cortex are observed, in vivo, by MRI at 4.7 Tesla. J. Magn. Reson. Imaging 2007;25:1210–1218. © 2007 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Sizing it up: Cellular MRI using micron-
Sizing it up: Cellular MRI using micron-sized iron oxide particles
✍ Erik M. Shapiro; Stanko Skrtic; Alan P. Koretsky 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 721 KB

## Abstract There is rapidly increasing interest in the use of MRI to track cell migration in intact animals. Currently, cell labeling is usually accomplished by endocytosis of nanometer‐sized, dextran‐coated iron oxide particles. The limitations of using nanometer‐sized particles, however, are tha

Quantification of superparamagnetic iron
Quantification of superparamagnetic iron oxide (SPIO)-labeled cells using MRI
✍ Ali M. Rad; Ali S. Arbab; A.S.M. Iskander; Quan Jiang; Hamid Soltanian-Zadeh 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 422 KB

## Abstract ## Purpose To show the feasibility of using magnetic resonance imaging (MRI) to quantify superparamagnetic iron oxide (SPIO)‐labeled cells. ## Materials and Methods Lymphocytes and 9L rat gliosarcoma cells were labeled with ferumoxides‐protamine sulfate complex (FE‐PRO). The cells we

Temporal and noninvasive monitoring of i
Temporal and noninvasive monitoring of inflammatory-cell infiltration to myocardial infarction sites using micrometer-sized iron oxide particles
✍ Yidong Yang; Yuhui Yang; Nathan Yanasak; Autumn Schumacher; Tom C.-C. Hu 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 343 KB

## Abstract Micrometer‐sized iron oxide particles (MPIO) are a more sensitive MRI contrast agent for tracking cell migration compared to ultrasmall iron oxide particles. This study investigated the temporal relationship between inflammation and tissue remodeling due to myocardial infarction (MI) us

Antibody-mediated cell labeling of perip
Antibody-mediated cell labeling of peripheral T cells with micron-sized iron oxide particles (MPIOs) allows single cell detection by MRI
✍ Erik M. Shapiro; Laura N. Medford-Davis; Tarek M. Fahmy; Cynthia E. Dunbar; Alan 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 296 KB

## Abstract Labeling cells with iron oxide is a useful tool for MRI based cellular imaging. Here it is demonstrated that peripheral rat T cells can be labeled in whole blood, __in vitro__, with streptavidin‐coated micron‐sized iron oxide particles (MPIOs), achieving iron concentrations as high as 6

Labeling of multiple cell lines using a
Labeling of multiple cell lines using a new iron oxide agent for cell tracking by MRI
✍ C. McFadden; C. L. Mallett; P. J. Foster 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 618 KB

Stem cells, cancer cells and immune cells were labeled by co‐incubation with a new ultra‐small iron oxide nanoparticle called Molday ION Rhodamine‐B (MIRB). Iron staining, fluorescence imaging, transmission electron microscopy and flow cytometry were used to assess cell viability, function and label