MR molecular imaging of the Her-2/neu receptor in breast cancer cells using targeted iron oxide nanoparticles

✍ Scribed by Dmitri Artemov; Noriko Mori; Baasil Okollie; Zaver M. Bhujwalla

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 610 KB
• Volume: 49
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.10406

✦ Synopsis

MR molecular imaging is an exciting new frontier in the biomedical applications of MR. One of the clinically relevant targets is the tyrosine kinase Her-2/neu receptor, which has a significant role in staging and treating breast cancer. In this study Her-2/neu receptors were imaged in a panel of breast cancer cells expressing different numbers of the receptors on the cell membrane. Commercially available streptavidin-conjugated superparamagnetic nanoparticles were used as targeted MR contrast agent. The nanoparticles were directed to receptors prelabeled with a biotinylated monoclonal antibody and generated strong T(2) MR contrast in Her-2/neu-expressing cells. The contrast observed in MR images was proportional to the expression level of Her-2/neu receptors determined independently with FACS analysis. In these experiments, iron oxide nanoparticles were attached to the cell surface and were not internalized into the cells, which is a major advantage for in vivo applications of the method.