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Mouse testis transcriptome revealed using serial analysis of gene expression

✍ Scribed by Junjie Yao; Takuya Chiba; Jun Sakai; Kunitaka Hirose; Mikio Yamamoto; Akiyuki Hada; Kazunao Kuramoto; Keiichi Higuchi; Masayuki Mori


Publisher
Springer-Verlag
Year
2004
Tongue
English
Weight
305 KB
Volume
15
Category
Article
ISSN
0938-8990

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The use of serial analysis of gene expression (SAGE) to determine gene expression profiles is increasing because the technique can provide absolute transcript numbers in a digital format and identify new genes. We developed a miniSAGE technique, which uses only 1 microg total RNA and reduces the amo