Mouse intestinal epithelial cells express the self superantigen Mis1

✍ Scribed by Dominique Kaiserlian; Karine Vidal; H. Robson MacDonald; Isabelle Grosjean

• Book ID: 102827662
• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 546 KB
• Volume: 23
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830231053

✦ Synopsis

In previous studies, we demonstrated that intestinal epithelial cells of the mouse small intestine could present exogenous antigen to specific CD4+ Tcell hybridomas. We now report on the abililty of normal enterocytes to present the self superantigen Mlsla. Enterocytes from Mlsl" but not from Mlslh strains stimulated interleukin-2 production through aVP6+ T cell hybridoma specific for Mlsla determinants. Antibody inhibition experiments showed that enterocytes presented MIS determinants via a major histocompatibility complex class 11- dependent mechanism. Furthermore, the ability of enterocytes to activate Vp6+ Mlsla-specific T cells was inhibited by monoclonal antibodies against the Orf protein encoded by an Mtv-7 provirus which is associated with Mlsla expression. These findings provide evidence for the first time that MIS determinants are expressed on normal enterocytes and support the theory of a possible role of these cells in extrathymic selection of Tcell receptor Vp repertoire of intraepithelial T lymphocytes.