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Mouse embryonic stem cells cultured under serum- and feeder-free conditions maintain their self-renewal capacity on hydroxyapatite

✍ Scribed by Nagai, Akiko; Hattori, Tatsuya; Hirose, Michiko; Ogura, Atsuo; Nozaki, Kosuke; Aizawa, Mamoru; Yamashita, Kimihiro


Book ID
122298601
Publisher
Elsevier Science
Year
2014
Tongue
English
Weight
1010 KB
Volume
34
Category
Article
ISSN
0928-4931

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## Abstract The proven pluripotency of ES cells is expected to allow their therapeutic use for regenerative medicine. We present here a novel suspension culture method that facilitates the proliferation of pluripotent ES cells without feeder cells. The culture medium contains polyvinyl alcohol (PVA