Mouse embryonic stem cell–based functional assay to evaluate mutations in BRCA2
✍ Scribed by Kuznetsov, Sergey G; Liu, Pentao; Sharan, Shyam K
- Book ID
- 109926092
- Publisher
- Nature Publishing Group
- Year
- 2008
- Tongue
- English
- Weight
- 478 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1078-8956
- DOI
- 10.1038/nm.1719
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A tk+/mouse embryonic stem (ES) cell line, designated 1G2, has been created in which one allele of the thymidine kinase (tk) gene was inactivated by targeted homologous recombination. This line is an analog of the mouse lymphoma tk+/-L5 178Y cell line, which is used widely to assess the mutagenicity
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