## Abstract To analyze the role of the classical and alternative pathways of complement activation in the effector phase of arthritis, we have induced arthritis in C3‐ and factor B (FB)‐deficient (C3^–/–^ and FB^–/–^) DBA/1J mice using well‐defined monoclonal IgG2b and IgG2a antibodies to type II c
Mouse Crry/p65 is a regulator of the alternative pathway of complement activation
✍ Scribed by Sharon Foley; Bin Li; Marlin Dehoff; Hector Molina; V. Michael Holers
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 459 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0014-2980
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## Abstract A simple, sensitive solid‐phase radioimmunoassay to quantitate the activation of the classical pathway of complement by rheumatoid factor (RF) is described. RF (purified, in serum or synovial fluid) was bound to reduced and alkylated IgG adsorbed to polyvinyl chloride microtiter plates
## Abstract ## Objective Collagen antibody–induced arthritis in mice exhibits a requirement for amplification by the alternative pathway of complement. Although the alternative pathway is activated by spontaneous hydrolysis, it is not known whether this pathway can also be initiated directly by Ig