✦ LIBER ✦

Mouse bone marrow-cultured macrophage as indicator cells for mouse and human migration inhibitory factor (MIF)

✍ Scribed by Marie-Luise Lohmann-Matthes; W. Domzig; H. -G. Meerpohl

Publisher: John Wiley and Sons
Year: 1977
Tongue: English
Weight: 394 KB
Volume: 7
Category: Article
ISSN: 0014-2980
DOI: 10.1002/eji.1830070817

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Macrophages cultured from mouse bone marrow served as excellent indicator cells for mouse as well as for human migration inhibitory factor (MIF) preparations. The tests were performed in the capillary system and showed high reproducibility. Using mouse or human MIF‐containing supernatants, we found an inhibition of 53 % compared with the controls. Similar results were obtained if concanavalin A‐stimulated human peripheral blood lymphocytes were mixed with mouse test macrophages in a ratio of 1:5 or 1:10.

📜 SIMILAR VOLUMES

Clonogenic methods in vitro for the enum

Clonogenic methods in vitro for the enumeration of granulocyte-macrophage progenitor cells (CFU-GM) in human bone marrow and mouse bone marrow and spleen

✍ Scott Cooper; Hal E. Broxmeyer 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 552 KB

Comparative analysis of the action of un

Comparative analysis of the action of unpurified mouse erythropoietin and human recombinant erythropoietin on erythroid and granulocytic-macrophagal precursor cells in semisolid mouse bone marrow cultures

✍ T. E. Manakova; M. A. Kapina 📂 Article 📅 1991 🏛 Springer US 🌐 English ⚖ 219 KB

The effect of mouse lung granulocyte-mac

The effect of mouse lung granulocyte-macrophage colony-stimulating factor and other colony-stimulating activities on the proliferation and differentiation of murine bone marrow cells in long-term cultures

✍ Neil Williams; Antony W. Burgess 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 English ⚖ 762 KB

## Abstract The roles of colony‐stimulating factors in long‐term bone marrow cultures were studied and compared. After single additions of high concentrations of unpurified colony‐stimulating activities to the cultures, rapid deterioration of the cultures was observed. This appears to result from c