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Motoneuron differentiation of immortalized human spinal cord cell lines

โœ Scribed by Ronghao Li; Silke Thode; Jiuying Zhou; Normand Richard; Jose Pardinas; Mahendra S. Rao; Dinah W.Y. Sah

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
832 KB
Volume
59
Category
Article
ISSN
0360-4012

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โœฆ Synopsis

Human motoneuron cell lines will be valuable tools for spinal cord research and drug discovery. To create such cell lines, we immortalized NCAM ฯฉ /neurofilament ฯฉ precursors from human embryonic spinal cord with a tetracycline repressible v-myc oncogene. Clonal NCAM ฯฉ / neurofilament ฯฉ cell lines differentiated exclusively into neurons within 1 week. These neurons displayed extensive processes, exhibited immunoreactivity for mature neuronspecific markers such as tau and synaptophysin, and fired action potentials upon current injection. Moreover, a clonal precursor cell line gave rise to multiple types of spinal cord neurons, including ChAT ฯฉ /Lhx3 ฯฉ /Lhx4 ฯฉ motoneurons and GABA ฯฉ interneurons. These neuronal restricted precursor cell lines will expedite the elucidation of molecular mechanisms that regulate the differentiation, maturation and survival of specific subsets of spinal cord neurons, and the identification and validation of novel drug targets for motoneuron diseases and spinal cord injury.

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