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Morphometry of articular cartilage: A stereological method using light microscopy

✍ Scribed by Paukkonen, Kari ;Selkäinaho, Kalevi ;Jurvelin, Jukka ;Helminen, Heikki J.

Book ID
102744077
Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
786 KB
Volume
210
Category
Article
ISSN
0003-276X

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✦ Synopsis

A stereological method is introduced for studying the light microscopic morphometry of articular cartilage. New parameters are presented for defining the three-dimensional structure of the articular cartilage. These include volume density of the lacunae and nuclei, numerical density of cells in 3-dimensional space, total number of cells, total volume of the lacunae in cartilage, volume and diameter of the lacuna and nucleus, the nucleusAacuna volume ratio, and the mean volume of the matrix per cell. Using the lateral tibial condyle of the knee joint of eight rabbits, we investigated tissue preparation, sampling, morphometry, and the conditions necessary for validity and precision of the estimators. The method proved easy to use, and the estimators were precise.

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