Morphological and functionnal differentiation of principal and intercalated cells in rabbit cortical collecting tubule primary culture
✍ Scribed by Marc, Jamous; Michel, Tauc; Philippe, Poujeol; Noémie, Koechlin
- Book ID
- 122263913
- Publisher
- Portland Press
- Year
- 1992
- Tongue
- English
- Weight
- 138 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0248-4900
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Transmembrane sodium transport pathways were studied in principal and intercalated cells of the isolated perfused rabbit cortical collecting duct. Intracellular electrolyte concentrations in individual collecting duct cells were measured by electron microprobe analysis during blockage of basolateral
K-dependent H+ extrusion was investigated using fluorescence techniques in rabbit cortical collecting tubules (CCTs). Experiments were performed in split-open tubules from normal animals exposed to the intracellular pH indicator 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF). This preparati