Morphological alterations induced by cold-shock in Neurospora crassa

✍ Scribed by Cristina Yoshiko Kawano; Suraia Said

• Book ID: 102680380
• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 689 KB
• Volume: 42
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/1521-4028(200212)42:6<381::aid-jobm381>3.0.co;2-8

✦ Synopsis

The effects of cold-shock on Neurospora crassa was here investigated. In cold-shock treated hyphae (6 h at 4 degrees C) the branching pattern was changed and dichotomy as well as a fan-like-pattern structure were temporarily detected at the tips. When exogenous Ca(2+) (500 mM) was added to the hyphae, these morphological alterations were not detected. In addition, the protein electrophoretic profile of cultures submitted to cold-shock was different from that of cultures kept at 30 degrees C. After 1 and 2 hours at 4 degrees C at least 5 bands were more intense than at 30 degrees C. The results suggest that cold-shock temporarily increases the synthesis of some proteins probably involved in the acclimatization process at 4 degrees C, and that the effects on morphology are probably due to damage to calcium influx.