Morphogenetic behaviour of two Streptomyces strains analyzed by the use of chemostats
✍ Scribed by Dr. S. Kretschmer
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 411 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0233-111X
No coin nor oath required. For personal study only.
✦ Synopsis
Streptomyces granaticolor E T H 7347 and Streptomyces hygroscopicus IMET J A 6599 were cultivated in Casamino acids-limited chemostats. Amino acids served as sole source of energy, carbon and nitrogen, but their limitation affected primarily the catabolism of the mycelia.At different specific growth rates (p) samples were wit,hdrawn. After staining the cell walls of the mycelia the average length of the cells (mycelial length per cross wall: L / C ) and of the hyplial growth units (mycelialength per branch : L / N ) were determined.
L/C was only slightly influenced by p. In S. granaticolor the cells were on average 11.5 pm and in S. hygroscopicus about 18.5 pm long.
However, L/N was dependent on p. Upon a shift from p = 0.6 to p == 0.1 L / N was reduced 1.2 fold in S. granaticolor and 1.8 fold in S. hygroscopicus. Especially growth rates below 0.2 stimulated branching. The opposite response of L/N to changes of p was, however, observed in glucoselimited chemostat cultures (RIESENBERG and BERGTER 1979). Thus, the average length of the