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Monovalent lectin as a novel tool for the resolution of microheterogeneity in glycoproteins

✍ Scribed by Avadhesha Surolia; P.S. Appukuttan; Debkumar Pain; B.K. Bachhawat

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
375 KB
Volume
105
Category
Article
ISSN
0003-2697

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✦ Synopsis

Microheterogeneous populations of the glycoprotein fetuin were found to bind molecules of the galactose-binding lectin ricin in proportion to the degree of desialation of the former and the number of galactose moieties exposed as a result. Monitoring the difference in molecular weight of the fetuin-ricin complexes formed from radioiodinated kin, native fetuin could be resolved into three broad heterogeneous groups.

Preparation of fetuin differing in Nacetylneuraminic acid content. For pre-1 Ricin being highly toxic should be handled carefully (5).

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