The ability of immature embryos of wheat (Triticum aestivum L.) to respond to tissue culture has been shown to involve the group 2 chromosomes. The available group 2 ditelosomic and nullisomic-tetrasomic lines of 'Chinese Spring' wheat were used to determine the chromosome arm location and chromosom
Monosomic analysis of tissue culture response in wheat (Triticum aestivumL.)
β Scribed by E. K. Kaleikau; R. G. Sears; B. S. Gill
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 711 KB
- Volume
- 78
- Category
- Article
- ISSN
- 0040-5752
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β¦ Synopsis
The ability of immature embryos of wheat (Triticum aestivum L.) to respond in cell culture was examined in crosses between the 'Wichita' monosomic series and a highly regenerable line, 'ND7532'. Segregation in disomic controls and 13 monosomic families showed a good fit to a monogenic ratio indicating a qualitative mode of inheritance. Segregation in the cross involving monosomic 2D showed a high frequency of regeneration (93.6%) and high callus growth rate (1.87 g/90 days) indicating that 2D is a critical chromosome. Modifying genes may be located on other chromosomes. Substitution of chromosomes from a low regenerable cultivar 'Vona' further indicated that the group 2 chromosomes, in particular chromosome 2D, possess genetic factors promoting callus growth and regeneration.
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