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Monophenolase activity of avocado polyphenol oxidase

✍ Scribed by Varda Kahn; Seymour H. Pomerantz


Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
724 KB
Volume
19
Category
Article
ISSN
0031-9422

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We describe a convenient and sensitive assay of polyphenol oxidase (PPO, EC 1.14 .18 .1 ) consisting of spectrophotometry at \(300 \mathrm{~nm}\) based on the stoichiometric reaction of cysteine with o-quinones produced during the enzymatic oxidation of phenols. The adduct formed exhibited spectral